Protein Expression & Enzymology

Recombinant Protein Expression Design, cloning and expression

We provide recombinant protein expression workflows to support enzyme generation, assay development, and mechanistic studies within glycoscience programmes. Services are delivered as fit-for-purpose packages aligned to project objectives and downstream use.

Expression strategy selection (scope-dependent; bacterial systems as standard)
Gene synthesis and construct planning support (where required)
Cloning and sequence verification workflows (scope-dependent)
Small-scale expression screening and optimisation
Scale-up planning for production batches (as required by scope)

Note: If you already have constructs or strains, we can work directly from supplied materials and focus on expression optimisation and production.

Enzyme Production for Glycoscience Workflows Heparinases, glycosidases and related enzymes

Enzymes are frequently central to glycosaminoglycan (GAG) analytics and functional workflows. We support enzyme production and qualification, including enzymes used for controlled depolymerisation and compositional mapping.

Production of enzymes used in GAG/carbohydrate analysis (scope-dependent)
Controlled depolymerisation and digestion workflows for downstream analytics
Enzyme performance profiling (activity, stability, and lot consistency)
Support for custom enzyme requests aligned to analytical needs
Integration with reagent supply via our eShop where appropriate

Protein Purification & Characterisation Purity, identity and stability

We provide purification and characterisation workflows to produce proteins and enzymes suitable for analytical use, assay development, or research applications.

Affinity purification workflows (tag-dependent)
Further polishing steps as required by application (fit-for-purpose)
Protein quantification and integrity assessment (e.g., SDS-PAGE)
Stability and storage condition evaluation (scope-dependent)
Documentation of purification and QC outcomes aligned to intended use

Enzymology & Activity Testing Kinetics, inhibition and robustness

We provide enzymology services to quantify and compare enzyme performance, support assay development, and enable robust application of enzymes within glycoscience analytical workflows.

Enzyme activity assays and time-course profiling
Kinetic analysis (e.g., Km/Vmax) where appropriate
Inhibition assays (IC50 determination and comparative ranking)
Substrate specificity studies and structure–activity relationships
Robustness testing (pH, salt, temperature, cofactors; scope-dependent)

Integration: Enzymology outputs can be strengthened by orthogonal analytics (e.g., MS/NMR) to confirm substrate conversion and product profiles.

Project-managed Delivery Coordination, oversight and reporting

Protein and enzymology work is often one component of broader programmes spanning structural analytics, bioassays and materials characterisation. TKBio provides scientific oversight, coordination and consolidated reporting to ensure that outputs are aligned to project objectives.

Definition of objectives, deliverables and acceptance criteria at project initiation
Coordination of expression, purification and activity testing workstreams
Alignment of enzyme outputs to downstream analytical or biological assays
Fit-for-purpose documentation and traceable data handling

Scope alignment: If your work has specific documentation, reporting, or material handling requirements, we will align feasibility and deliverables at project initiation.

Link to reagents: Where appropriate, enzymes and related materials may also be supplied via our eShop to support repeatable workflows and inter-laboratory comparability.

Enquiries: enquiries@tkbio.co.uk