Enzymatic & Kinetic Assays Activity, inhibition and mechanism
We deliver enzymatic and biochemical assays to support glycoscience programmes, including assay design, optimisation, and clear interpretation of results.
- Enzyme activity assays (glycosidases, lyases and related systems)
- Kinetic analysis (initial rate, Km/Vmax, time-course profiling)
- Inhibition assays (IC50 determination and comparative profiling)
- Substrate specificity studies and structure–activity relationships
- Assay miniaturisation and plate-based workflows where appropriate
Binding & Interaction Studies Glycan–protein and biomolecule interactions
We support studies of biomolecular interactions involving carbohydrates and glycosaminoglycans, including comparative binding assessments and structure–function questions.
- Glycan–protein interaction studies (fit-for-purpose formats)
- Competitive binding and displacement experiments
- Affinity ranking across variants, sources or batches
- Orthogonal analytical support (e.g., MS/NMR/SEC) to contextualise biological findings
Immunoassays & Protein Analytics ELISA, multiplex and protein methods
We provide immunoassay and supporting protein analytical services either directly or on a project-managed basis, depending on the specific scope and documentation needs.
- ELISA and plate-based immunoassays
- Multiplex assays where appropriate
- Protein quantification (e.g., BCA/Bradford)
- SDS-PAGE and Western blotting
- Stability and degradation assessments (fit-for-purpose)
LC-MS Based Bioanalysis Quantification and profiling
LC-MS workflows can be deployed for quantitative bioanalysis and targeted profiling in support of glycoscience and complex biomolecule programmes.
- Targeted LC-MS/MS quantification (fit-for-purpose)
- High-resolution MS profiling where required
- Sample preparation development and recovery assessment
- Matrix and interference assessment aligned to intended use
- Result interpretation in the context of orthogonal analytics (e.g., NMR/SEC)
Method Development & Fit-for-purpose Validation Rigour aligned to use-case
We recognise that different programmes require different levels of method control and documentation. We therefore design method development and performance assessment to match the decision-making context, from exploratory research through to quality-focused investigations.
- Assay development, optimisation and robustness checks
- Performance assessments (precision, accuracy, linearity, sensitivity) where required
- Reference material selection, controls and acceptance criteria definition
- Data packages structured for research, comparability and QC contexts
- Method transfer support and cross-platform comparability studies